Radicals and antioxidants updating a personal view Cam4 germany free
PCA showed that PC1 and PC2 explained 90.43% of the total variability of the antioxidant data.
Most of the seeds showed omega-3, omega-6, and omega-9 fatty acids at significant concentrations, with two PCs explaining 93.80% of the total variance of the fatty acid contents.
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Several methods have been developed to measure the antioxidant capacity of fruits.The split ratio was 1 : 80 and 2 μL of the samples was injected in triplicate.The detector and injection port were set at 240°C and the column was set at 185°C for 7.5 min.The fruits were washed with tap water and the pulps, peels, and seeds were removed manually, chopped, and then homogenized in a multiprocessor to uniformity before analysis. dulcis pulp and peels were evaluated together because it was not possible to separate them. edulis peels were discarded., 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,4,6-tris (2-pyridyl)-s-triazine (TPTZ), and methanol and acetonitrile (HPLC grade) were purchased from Sigma Chemical Co. All solvents and chemicals were of analytical grade.The extracts were prepared using 10 g (per part of the fruit) in 100 m L of methanol under magnetic stirring for 4 h according to the method developed by Santos et al. After filtration, the extracts were concentrated under reduced pressure at 40°C. After gently mixing and then resting the reaction solutions at room temperature for 30 min, the absorbance was measured at 517 nm.